Measurement science underpins the broadest range of scientific disciplines in the natural world, and very often those measurements are required of complex mixtures. To understand the nature of such mixtures or matrices we need the means to investigate at the component level. This module will provide students with the comprehensive knowledge-base in methodology and advanced analytical techniques required for fit-for-purpose quantitative and qualitative analyses of complex mixtures, or compounds within complex environmental, atmospheric, chemical or biological matrices. Within this, approaches to sample preparation, the fundamental principles of advanced separation science, mass spectrometry detection and optical measurement techniques, will be covered at a level of technical detail that ensures knowledge in metrology gained can be applied across strategic application areas that are important in the natural world.
This course will introduce you to the analytical challenges faced when studying complex mixtures, whether through direct analysis or combining a number of complementary techniques, and whether for qualitative or quantitative purposes.
Through case studies, and examples taken from peer-reviewed literature, you will have the opportunity to explore a wide range of application areas that stem from a set of common principles, and learn how to critically think through analytical approaches so as to tailor them to meet different challenges.
Module learning outcomes
Subject content
To develop a knowledge-base of:
The diversity of complex mixtures in the natural world
The dynamic range of compounds within matrices, and the consequences to analytical science
Advanced separation science for simplifying mixtures of liquids or gases prior to detection
Ion mobility separation mass spectrometry for filtering ions based on shape, and obtaining a measure of collisional cross section to indicate size.
Advanced mass spectrometry for structure determination, including high resolution and accurate mass measurement, and technologies for tandem mass spectrometry
Optical techniques for highly selective measurements in complex mixtures
The application of metrology (qualitative and quantitative measurement science) to complex mixtures in the natural world including atmospheric, biological, chemical, environmental matrices.
Analytical strategies and method validation to ensure data is fit for intended purpose
Academic and graduate skills
Will be able to determine appropriate analytical methods for different applications within environmental, atmospheric, chemical or biological matrices.
Will be able to design and validate experimental procedures to ensure fit-for-purpose methods are used and outcomes result.
Will develop critical and applied thinking through critical assessments of some research papers or case studies
Module content
Introduction to the course, highlighting hot topics in York
1 lecture JAM
Measurement science across the phases of state
7 lectures KEHP
Sample strategies and approaches for sample preparation
Qualitative and quantitative analysis
Method validation
Analytical strategies for method development and problem solving
Case studies
Spectroscopic techniques and sensors
7 lectures PME
Cavity and open path absorption spectroscopy (incl. DOAS)
Multidimensional chromatography with mass selective detection 7 lectures JFH/Rosie Chance/Marvin Shaw.
2D GC
2D LC
GC-LC
Chemical ionisation mass spectrometry
Proton transfer reactor mass spectrometry
Principles of linear and reflecttron Time-of-Flight mass spectrometry
Case studies
High resolution mass spectrometry and accurate mass measurement 6 lectures JAM
Fourier transform mass spectrometry
Tandem mass spectrometry
Ion mobility separation with mass spectrometry
Elemental analysis and isotope ratio mass spectrometry
Direct analysis mass spectrometry
Case studies
Indicative assessment
Task
% of module mark
Online Exam -less than 24hrs (Centrally scheduled)
100
Special assessment rules
None
Additional assessment information
Online open book 2 hour exam consisting of 2 x 20 mark questions. To be submitted via the VLE within the 24h assessment window.
Indicative reassessment
Task
% of module mark
Online Exam -less than 24hrs (Centrally scheduled)
100
Module feedback
Exam results with per-question breakdown are returned to the students via supervisors within 5 weeks (as per special approval by the University Teaching Committee). Outline answers are made available via the Chemistry web pages or vle when the students receive their marks, so that they can assess their own detailed progress/achievement.
Lecturers encouraged to put short quizzes on VLE, with opportunity to provide experience and feedback at data manipulation and interpretation where relevant
Indicative reading
To be provided by individual lecturers and will include 2 papers from literature exemplifying taught material