Protein Production equipment
Our staff provide fully-assisted technical support for the infrequent user and the facility is an excellent equipment resource for the experienced user.
Find out more about the facilities and services we offer at the Protein Production Laboratory, or contact us for more information.
What we offer
Contact us
Dr Jared Cartwright
Protein Production Lab Head
jared.cartwright@york.ac.uk
01904 328797
Technology Facility, Department of Biology, University of York, Wentworth Way, York, YO10 5DD UK
General enquiries to: btf-pp-group@york.ac.uk
Proteases
The Protein Production labs prepare and supply H3c (PreScission) and TEV protease for general R&D applications. Both proteases are produced in E. coli using TFPP engineered vectors to give near-native HIS-tagged protease.
The expression procedure uses the Im9 solubility tag (which is self-cleaved) to ensure high-level production. The near native proteases are then purified using Ni2+-affinity chromatography and supplied at both high purity and specific activity.
Contact us for a quotation or supply
Our facilities
| Process | Instrument | Description |
|---|---|---|
| Robotics | HAMILTON | High-spec liquid handling robotics |
| Fermentation | APPLIKON 70 litre (SIP) | Microbial (steam-in-place) |
| APPLIKON 2-7 litre glass (x4) | Microbial / Cell culture | |
| APPLIKON 20 litre SUBs (x1) | Cell culture | |
| APPLIKON 0.5 litre MiniBio (x4) | Microbial / Cell culture | |
| Separations | Hollow-fibre separations | Bacteria/insect |
| HERAEUS | Continuous flow centrifugation | |
| Incubation | SCIQUIP (x5) | Cooled Microbial Incubators |
| INFORS Multitron (x2) | Insect Shaker Incubators | |
| Incubation | THERMO (x2) | Static Cell Culture Incubators |
| EPPENDORF | Mammalian Shaker Incubator | |
| Cell Lysis | COLE PALMER | Sonication |
| CONSTANT SYSTEMS | Cell Disruptor | |
| Centrifugation | BECKMAN | Process-Scale Centrifugation |
| SORVAL Evolution | High-Speed Centrifugation | |
| BECKMAN | Ultracentrifugation | |
| Purification | BIORAD | NGS Quest |
| ÄKTA Pure | Cold cabinet | |
| ÄKTA Purifier 10 | HPLC - Preparative | |
| ÄKTA Xpress (x3) | Cold cabinet | |
| Microbiology | Class II hood | General microbiology |
| Tissue Culture | Insect | Baculovirus expression |
| Mammalian | HEK293 expression | |
| Imaging | SYNGENE | General Gel Documentation |
| THERMO | iBRIGHT FL1000 |
Contact us
Dr Jared Cartwright
Protein Production Lab Head
jared.cartwright@york.ac.uk
01904 328797
Technology Facility, Department of Biology, University of York, Wentworth Way, York, YO10 5DD UK
General enquiries to: btf-pp-group@york.ac.uk