Professor James Chong

Research

Anaerobic applied systems biology

Anaerobic microbes were among the first forms of life to inhabit our planet. They continue to occupy a wide range of environments (anywhere oxygen concentrations are low...) and have developed a wide repertoire of unique biochemical pathways.

Methanogenic archaea are an increasingly economically important group of microorganisms. A series of unique biochemical pathways means that these microbes are able to synthesise methane, the flammable component of natural gas, from basic components such as carbon dioxide and hydrogen. On a global scale, methanogens are responsible for the synthesis of approximately a billion tonnes of methane per year.  In addition to their potential to contribute to climate change, methanogens are also a key component in the decomposition of organic matter via anaerobic digestion (AD). AD has the potential to produce substantial quantities of renewable energy from waste. This technology is already used to dispose of food waste and recover resources from wastewater sludge. My group is using molecular methods to understand how anaerobic microbial communities, which include methanogens, interact with each other and respond to their environment.  In collaboration with industrial partners, we are measuring the dynamics of the microbial consortia involved in anaerobic digestion. We have recently commissioned System-60: 60 automatically-fed 5L AD systems that allow us to perform replicated experiments to investigate and model community changes in process scale AD. In collaboration with the Biorenewables Development Centre at York we have designed and commissioned a suite of 30L AD reactors equipped with analytical facilities to monitor the physico-chemical changes in these systems.

System-60

Our lab also houses comprehensive facilities for the growth and genetic manipulation of methanogens. We are one of a handful of groups world-wide that have developed and used genetic tools to manipulate methanogen (our organism of choice is Methanococcus maripaludis).  Using these facilities we are able to isolate, grow and characterise novel methanogens and other anaerobes for use in real-world applications.