Department of Biology
Dr Laura Wiggins - University of Sheffield
Wednesday 6 November 2024, 1.00PM to 2pm
Speaker(s): Dr Laura Wiggins - University of Sheffield
No labels, just discoveries: the power of ptychography for single-cell characterisation
With phenotypic heterogeneity in whole cell populations widely recognised, the demand for quantitative and temporal analysis approaches to characterise single-cell morphology and dynamics has increased. To address this, our team developed CellPhe, a pattern recognition toolkit for the unbiased characterisation of cellular phenotypes within time-lapse videos. CellPhe has demonstrated its
value in quantifying single-cell responses to drug treatment, facilitating imaging of co-cultures and identifying cell-cell signalling events. However, the complexities of biological data sets and evolution of new imaging technologies call for even more advancedanalysis solutions. Here we present CellPhe 2, which builds upon our original cell phenotyping toolkit by incorporating new analysis capabilities for population-level modelling, tracklet analysis and interoperability with existing open-source cell segmentation and tracking
software. CellPhe 2 is designed to facilitate analysis of complex data sets from multi-channel fluorescence images through to densely populated samples where conventional segmentation approaches underperform.
Location: B/K/018 Dianne Bowles Lecture Theatre