Looking for a needle in a haystack: searching for substrates of the N-end rule of targeted protein degradation using genetics and proteomics

In eukaryotic cells, controlled degradation plays a pivotal role in determining protein abundance throughout signalling and development. The N-end rule pathway is aspecialised type of targeted protein degradation which links the half-life of a protein to the identity of its amino (N-) terminal residue. Whilst analysis of mutants impaired in different pathway components has provided insight into the physiological functions of the N-end rule, knowledge regarding the identity of substrates and the impact of this pathway on the proteome is limited. In this talk I will discuss the use of genetics, quantitative proteomics and N-terminal peptide enrichment to identify substrates and downstream targets of the pathway of the N-end rule in plants, an endeavour that led us from seed germination, through lipid metabolism to discoveries in gas sensing and beyond.

More on Professor Frederica Theodulou.  

For further info please contact the host: mauro.rinaldi@york.ac.uk