Regulation of ESCRT-III-dependent nuclear envelope regeneration during mitotic exit

In addition to its well characterised role in degradative endosomal sorting and budding of enveloped retroviruses, the ESCRT-III complex is repurposed during division to perform a number of membrane remodelling events including regeneration of the nuclear envelope and the abscission phase of cytokinesis. Nuclear envelope regeneration requires transient assembly of ESCRT-III at the nuclear periphery to seal small holes in this organelle and so allow nucleocytoplasmic compartmentalisation. ESCRT-III assembly is initiated through interaction of the ER-localised ESCRT-III protein, CHMP7, with the inner nuclear membrane protein LEM2. I will discuss how this interaction is restricted during interphase and how cell cycle control programmes regulate the behaviour of CHMP7 during mitosis to allow for specific regeneration of the nuclear envelope.